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atcc 14019 strain  (ATCC)


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    Structured Review

    ATCC atcc 14019 strain
    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV <t>ATCC</t> <t>14019</t> (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
    Atcc 14019 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vaginalis+atcc+14019+strain/Gardnerella+vaginalis%3B+317/pmc12926587-166-20-20
    Average 95 stars, based on 87 article reviews
    atcc 14019 strain - by Bioz Stars, 2026-09
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    1) Product Images from "The proteinaceous biofilm of Gardnerella vaginalis enables a novel enzymatic therapy for bacterial vaginosis"

    Article Title: The proteinaceous biofilm of Gardnerella vaginalis enables a novel enzymatic therapy for bacterial vaginosis

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2026.100346

    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV ATCC 14019 (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
    Figure Legend Snippet: Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV ATCC 14019 (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.

    Techniques Used: Infection, Concentration Assay, Control

    Related Articles

    Amplification:

    Article Title: The presence of the putative Gardnerella vaginalis sialidase A gene in vaginal specimens is associated with bacterial vaginosis biofilm
    Article Snippet: .. The design of the primer set for amplification of the putative G . vaginalis sialidase A gene was based on previous work by Lopes dos Santos Santiago et al. [ ] and on the sequence of sialidase A (NZ_ ACGF02000001.1) from the fully sequenced G . vaginalis ATCC 14019 strain (reference genome for the Human Microbiome Project, Baylor College of Medicine, Houston, TX). .. It should be noted that although the G . vaginalis ATCC 14019 reference strain originates from a woman diagnosed with BV and includes the putative sialidase A gene, it does not produce sialidase in laboratory cultures [ , ].

    Sequencing:

    Article Title: The presence of the putative Gardnerella vaginalis sialidase A gene in vaginal specimens is associated with bacterial vaginosis biofilm
    Article Snippet: .. The design of the primer set for amplification of the putative G . vaginalis sialidase A gene was based on previous work by Lopes dos Santos Santiago et al. [ ] and on the sequence of sialidase A (NZ_ ACGF02000001.1) from the fully sequenced G . vaginalis ATCC 14019 strain (reference genome for the Human Microbiome Project, Baylor College of Medicine, Houston, TX). .. It should be noted that although the G . vaginalis ATCC 14019 reference strain originates from a woman diagnosed with BV and includes the putative sialidase A gene, it does not produce sialidase in laboratory cultures [ , ].



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    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV <t>ATCC</t> <t>14019</t> (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
    Atcc 14019 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV <t>ATCC</t> <t>14019</t> (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
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    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV <t>ATCC</t> <t>14019</t> (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
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    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV <t>ATCC</t> <t>14019</t> (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
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    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV <t>ATCC</t> <t>14019</t> (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.
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    Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV ATCC 14019 (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.

    Journal: Biofilm

    Article Title: The proteinaceous biofilm of Gardnerella vaginalis enables a novel enzymatic therapy for bacterial vaginosis

    doi: 10.1016/j.bioflm.2026.100346

    Figure Lengend Snippet: Vaginal epithelial cell morphology (a–d) and changes in 16S rRNA gene copy number of G. vaginalis and lactobacilli in the vaginas of GV-infected mice following LasA or antibiotic treatment (e,f). Vaginal secretion smears from GV JNFY14-infected mice showing epithelial cells and adherent GV cells. Insets highlight zoomed-in regions (scale bar, 10 μm; inset bar, 2 μm). (a) Uninfected epithelial cells; (b) GV-infected vaginal epithelial cells without treatment; (c) GV-infected vaginal epithelial cells after LasA treatment; (d) GV-infected vaginal epithelial cells after ERM treatment. Arrows indicate GV cells adhered to epithelial cell surface. Biomass changes of G. vaginalis and lactobacilli populations after treatment in the vagina of mice infected with GV JNFY14 (e) or GV ATCC 14019 (f). Successfully infected mice were divided into 3 groups and treated once a day for 6 days: Group 1 was treated with 20 μg/mL LasA, group 2 was treated with the same concentration of antibiotics (ERM for strain JNFY14 and MTZ for strain ATCC 14019, respectively); and the control group was treated with an equal volume of 50 mM PBS buffer (pH 7.0). Statistical analysis was carried out using GraphPad Prism 9.5.1. ∗∗, p < 0.05; ∗∗∗, p < 0.01; ∗∗∗∗, p < 0.005.

    Article Snippet: This observation may be attributed to the JNFY14 strain's better adaptation to the vaginal environment of mice compared to the ATCC 14019 strain.

    Techniques: Infection, Concentration Assay, Control